primary normal human dermal fibroblasts hdf  (PromoCell)

Journal: International Journal of Molecular Sciences

Article Title: A Simple Ionic-Gelation Method for Chitosan Nanoparticle Synthesis and Standardized Protocols for Biological Safety Assessment: Antibacterial Activity, Phytotoxicity, and Biocompatibility

doi: 10.3390/ijms27083673

Figure Lengend Snippet: Cell viability of HDF cells treated with Ch NPs at concentrations of 2.5–160 µg/mL for 24 h. Viability was determined using the MTT assay and expressed as mean ± SE (n = 3). Different letters indicate statistically significant differences ( p < 0.05).

Article Snippet: The biocompatibility of Ch NPs was evaluated using primary HDF (ATCC ® PCS-201-012TM [ ], American Type Culture Collection, Manassas, VA, USA) and HaCaT cells (Cat. No. T0020001, AddexBio, San Diego, CA, USA).

Techniques: MTT Assay

Journal: Biomedicines

Article Title: Mesenchymal Stem Cell–Derived Exosomes Mitigate Cutaneous Radiation Injury Through Coordinated Modulation of DNA Repair, Stress, and Inflammatory Gene Programs

doi: 10.3390/biomedicines14040811

Figure Lengend Snippet: Expression of early damage and stress-response genes ( a ) FDXR , ( b ) GADD45A , ( c ) SESN1 , and ( d ) GDF15 in irradiated HDFs (10 Gy) treated with exosomes (1 × 10 9 particles/mL) derived from BM-MSCs (BM-IR) and UC-MSCs (UC-IR), measured by qPCR. Gene expression was normalized to GAPDH and expressed as fold change relative to the non-irradiated Vehicle control at each timepoint. Data are shown as mean ± SD of three technical replicates from a representative experiment performed using a single HDF donor. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by ordinary one-way ANOVA followed by Dunnett’s post hoc test, comparing each treatment group to the Vehicle-IR control at the corresponding timepoint.

Article Snippet: Primary human dermal fibroblasts (HDFs) were purchased from PromoCell GmbH (SKU: C-12302; Heidelberg, Germany).

Techniques: Expressing, Irradiation, Derivative Assay, Gene Expression, Control

Journal: Biomedicines

Article Title: Mesenchymal Stem Cell–Derived Exosomes Mitigate Cutaneous Radiation Injury Through Coordinated Modulation of DNA Repair, Stress, and Inflammatory Gene Programs

doi: 10.3390/biomedicines14040811

Figure Lengend Snippet: Expression of DNA repair and antioxidant genes ( a ) DDB2 , ( b ) RNF8 , and ( c ) SOD1 in irradiated HDFs (10 Gy) treated with exosomes (1 × 10 9 particles/mL) derived from BM-MSCs (BM-IR) and UC-MSCs (UC-IR), measured by qPCR. Gene expression was normalized to GAPDH and expressed as fold change relative to the non-irradiated Vehicle control at each timepoint. Data are shown as mean ± SD of three technical replicates from a representative experiment performed using a single HDF donor. * p < 0.05, ** p < 0.01, *** p < 0.001, by ordinary one-way ANOVA followed by Dunnett’s post hoc test comparing each treatment group to the Vehicle-IR control at the corresponding timepoint.

Article Snippet: Primary human dermal fibroblasts (HDFs) were purchased from PromoCell GmbH (SKU: C-12302; Heidelberg, Germany).

Techniques: Expressing, Irradiation, Derivative Assay, Gene Expression, Control

Journal: Biomedicines

Article Title: Mesenchymal Stem Cell–Derived Exosomes Mitigate Cutaneous Radiation Injury Through Coordinated Modulation of DNA Repair, Stress, and Inflammatory Gene Programs

doi: 10.3390/biomedicines14040811

Figure Lengend Snippet: Expression of cell-cycle regulation and proliferation genes ( a ) CDKN1A , ( b ) CDKN2A , ( c ) MKI67 , ( d ) H2AFX , and ( e ) VEGFA in irradiated HDFs (10 Gy) treated with exosomes (1 × 10 9 particles/mL) derived from BM-MSCs (BM-IR) and UC-MSCs (UC-IR), measured by qPCR. Gene expression was normalized to GAPDH and expressed as fold change relative to the non-irradiated Vehicle control at each timepoint. Data are shown as mean ± SD of three technical replicates from a representative experiment performed using a single HDF donor. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by ordinary one-way ANOVA followed by Dunnett’s post hoc test, comparing each treatment group to the Vehicle-IR control at the corresponding timepoint.

Article Snippet: Primary human dermal fibroblasts (HDFs) were purchased from PromoCell GmbH (SKU: C-12302; Heidelberg, Germany).

Techniques: Expressing, Irradiation, Derivative Assay, Gene Expression, Control

Journal: Biomedicines

Article Title: Mesenchymal Stem Cell–Derived Exosomes Mitigate Cutaneous Radiation Injury Through Coordinated Modulation of DNA Repair, Stress, and Inflammatory Gene Programs

doi: 10.3390/biomedicines14040811

Figure Lengend Snippet: Expression of inflammatory response genes ( a ) IL-6 and ( b ) TNFAIP3 in irradiated HDFs (10 Gy) treated with exosomes (1 × 10 9 particles/mL) derived from BM-MSCs (BM-IR) and UC-MSCs (UC-IR), measured by qPCR. Gene expression was normalized to GAPDH and expressed as fold change relative to the non-irradiated Vehicle control at each timepoint. Data are shown as mean ± SD of three technical replicates from a representative experiment performed using a single HDF donor. * p < 0.05, ** p < 0.01, **** p < 0.0001 by ordinary one-way ANOVA followed by Dunnett’s post hoc test, comparing each treatment group to the Vehicle-IR control at the corresponding timepoint.

Article Snippet: Primary human dermal fibroblasts (HDFs) were purchased from PromoCell GmbH (SKU: C-12302; Heidelberg, Germany).

Techniques: Expressing, Irradiation, Derivative Assay, Gene Expression, Control

Journal: Bioactive Materials

Article Title: Multifactorial bioengineering in the tendon context to maintain tenocyte phenotype and to direct dermal fibroblasts towards tenogenic lineage

doi: 10.1016/j.bioactmat.2025.11.008

Figure Lengend Snippet: Immunofluorescence intensity analysis for COL I, III, IV, V, VI, COL I/COL III ratio, TNMD and aSMA in hTCs and hDFs after 4, 7 and 10 days of culture under PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions. (∗p < 0.05 vs respective PLLA group at the same time point; ‡p < 0.05 vs respective + TGFB2 group at the same time point; #p < 0.05 vs. respective + MMC group at the same time point; §p < 0.05 between cell types at the same time point and condition; &p < 0.05 vs. respective condition at day 4). N = 3.

Article Snippet: Cryopreserved hDFs (PCS-201-012) were purchased from the American Type Culture Collection (ATCC, United Kingdom).

Techniques: Immunofluorescence

Journal: Bioactive Materials

Article Title: Multifactorial bioengineering in the tendon context to maintain tenocyte phenotype and to direct dermal fibroblasts towards tenogenic lineage

doi: 10.1016/j.bioactmat.2025.11.008

Figure Lengend Snippet: Principal component analysis ( A ) of the quantitative proteomic data obtained from hTCs and hDFs after 10 days of culture under TCP, PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions, as well as from TT samples. Venn diagram representing the top 100 protein hits in TT group vs. hTCs ( B ) and hDFs ( C ) cultured under PS, PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions. Gene ontology analysis ( D ) of the top 100 protein hits in TT group vs. hTCs and hDFs cultured under PS, PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions. N = 3.

Article Snippet: Cryopreserved hDFs (PCS-201-012) were purchased from the American Type Culture Collection (ATCC, United Kingdom).

Techniques: Cell Culture

Journal: Bioactive Materials

Article Title: Multifactorial bioengineering in the tendon context to maintain tenocyte phenotype and to direct dermal fibroblasts towards tenogenic lineage

doi: 10.1016/j.bioactmat.2025.11.008

Figure Lengend Snippet: Gene set enrichment analysis of the quantitative proteomic profile of hDFs cultured for 10 days under PLLA ( A ), +TGFB2 ( B ), +MMC ( C ), and +MMC + TGFB2 ( D ) conditions. All contrasts were performed against hDFs cultured for 10 days under TCP conditions. A gene set containing all protein hits in TT group vs hDFs TCP group (tendon signature) was used for all analyses. Venn diagram summarizing the different core enrichment proteins identified in PLLA, +TGFB2, +MMC and +MMC + TGFB2 groups by means of gene set enrichment analysis ( E ). GO analysis of the core enrichment proteins identified in +MMC + TGFB2 group by means of gene set enrichment analysis ( F ). (NES: normalised enrichment score). N = 3.

Article Snippet: Cryopreserved hDFs (PCS-201-012) were purchased from the American Type Culture Collection (ATCC, United Kingdom).

Techniques: Cell Culture

Journal: Bioactive Materials

Article Title: Multifactorial bioengineering in the tendon context to maintain tenocyte phenotype and to direct dermal fibroblasts towards tenogenic lineage

doi: 10.1016/j.bioactmat.2025.11.008

Figure Lengend Snippet: Heatmap displaying the quantitative matrisome of hTCs and hDFs cultured under PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions for 10 days. Hierarchical clustering was applied to all samples. The heatmap displays the z-scores for every matrisome protein identified, being these grouped into the different matrisome categories. Both hTCs and hDFs clustered first in relation to TGFB2 treatment (alone or in combination with MMC), and ultimately in relation with the experimental conditions to which these were subjected. The + MMC + TGFB2 experimental condition exerted the highest changes into the quantitative matrisome of hTCs and hDFs, increasing ECM protein levels throughout all the categories of the matrisome. N = 3.

Article Snippet: Cryopreserved hDFs (PCS-201-012) were purchased from the American Type Culture Collection (ATCC, United Kingdom).

Techniques: Cell Culture